Investigation of medicinal plants response to fertilizers is one of the main issues about their cultivation. In this regard in current experiments the response of isabgol (Plantago ovata) and black seed (Nigella sativa) to some fertilizer resources was evaluated in two separated experiments at research field of Sarayan faculty of agriculture, university of Birjand, Iran during 2015-16. In the first experiment the influence of vermiCOMPOST (2. 5 and 5 ton. ha-1), cow manure (10 and 20 ton. ha-1) and humic acid application (1 and 2 kg. ha-1) combined with control (no-fertilizer) was studied on some qualitative and quantitative indices of isabgol based on a randomized complete block design with three replications. In the second study the effects of SULPHUR (0, 150 and 300 kg. ha-1), Thiobacillus bacteria (50: 1 weight ratio of sulfur and bacteria) and cow manure (0 and 30 ton. ha-1) was evaluated on yield and yield components of black seed. Cow manure, Thiobacillus and SULPHUR were considered as main, sub and sub-sub factors, respectively. Results showed that the effect of fertilizer type was significant only on number of tiller, number of spike and SPAD reading in isabgol. The highest tiller and spike (5. 6 and 48. 5 number, respectively) was obtained by 2 kg. ha-1 humic acid application, while the lowest (3. 8 and 31. 6 number, respectively) was belonged to control treatment. Application of cow manure and humic acid were increased partially the swelling factor of seeds. In addition, the highest seed yield (3. 76 g per plant or 1038 kg. ha-1) was obtained at the rate of 2 kg. ha-1 humic acid usage. In the second experiment, the impact of soil fertilizer type was not significant on black seed medicinal plant growth indices. However, almost all of studied criteria were improved by cow manure consumption, so that finally the seed yield of the plant was 292 and 243 kg. ha-1 in application and no-application cow manure treatments, respectively. Thiobacillus had no significant effect on all of studied indices and only increased seed yield per hectare by 5 kg. Overall, our results revealed that isabgol and black seed are two low-input medicinal plants in terms of nutrients requirements.